* Whether there is anything in their stomach or guts (reasons for this should be fairly straightforward!)
* Whether they have any foreign proteins or other components in their blood, which often 'accidentally' get absorbed into our bloodstream from food. Prana would never create a protein usually only found in a cow's body, but foreign and harmful to a human body, for example. (Usually our bodies recognise such foreign proteins as invaders, and our immune systems destroy them. However they can still be found in our blood.)
* For a long-term breatharian, a test would be whether they have the same amount of toxins in their body as another person of the same age living in the same area. Toxins such as heavy metals, pesticides, etc, accumulate on or in food, and when we eat food they often just accumulate in our flesh as our bodies are not good at getting rid of them. While there are some of these in the air, which a breatharian would breathe in, the vast majority come from food, so it would be expected a long-term breatharian would have far less than a person who eats normal amounts of food.
* I am not a doctor, and only understand the basics of how our bodies work. A doctor could no doubt come up with other factors to test for, based on the claims of breatharians having very pure bodies, receiving energy directly, and without producing toxins.
A caution for doctors who want to test for breatharianism:
It would also be necessary to consider the possibility the test subject actually has a diet of mainly fruit, or liquid (juice etc), or a raw plant food diet, or vegan diet - when done well, people can live on these indefinitely and very healthily, but people on these diets would have different components and levels of impurities in their blood, compared with the majority of people on traditional meat-and-veg diets.
Therefore, in any tests comparing a breatharian with people who eat, perhaps the control subjects could be people who eat only raw plant food (raw foodists), or similar fairly pure diets with low levels of toxins.
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